Mouse Anti-Human CD14

• CD14 (Cluster of Differentiation 14) is a receptor protein predominantly found on macrophages, playing a vital role in the innate immune system. It exists in two forms:

  1. Membrane-bound CD14 (mCD14): Anchored via a glycosylphosphatidylinositol (GPI) tail (48 kDa).
  2. Soluble CD14 (sCD14): Produced either through mCD14 shedding or secretion from intracellular vesicles (56 kDa).

  CD14 binds lipopolysaccharides (LPS) from gram-negative bacteria, facilitating their recognition by Toll-like receptor 4 (TLR4), a critical step in immune activation and inflammation. Research applications include studies on infection mechanisms, immune responses, and inflammation-related diseases.

• • Host Species: Mouse
  • Target Species: Human
  • Conjugation: Unconjugated
  • Clonality: Monoclonal
  • Isotype: IgG
  • Form: Liquid

  Purification

  This antibody is purified via immunoaffinity chromatography using protein G coupled to Sepharose 4B, ensuring high specificity and purity.

  Immunogen

  The immunogen is a KLH-conjugated synthetic peptide derived from the human CD14 protein, designed to ensure optimal immune response during antibody production.

• • Storage Instructions:
    • Store at -20°C upon receipt.
    • Avoid repeated freeze-thaw cycles.
    • The product is stable for several weeks at 4°C as an undiluted liquid.
  • Storage Buffer:
    • Phosphate buffered saline (pH 7.4) containing stabilizer and ≤0.09% sodium azide.

  For extended storage, aliquot and freeze at -20°C or below. If the product is not completely clear after standing at room temperature, centrifuge before use.

This antibody is suitable for the following research applications:

• Western Blotting (WB)
• Immunohistochemistry (Paraffin/Frozen) (IHC-P/IHC-F)
• Immunofluorescence (IF)
• Dot Blot
• Immunomicroscopy
• ELISA

Recommended starting dilutions:

• Western Blotting: 3–5 µg/ml
• IHC (Paraffin/Frozen): 5–10 µg/ml
• Immunofluorescence: 5–10 µg/ml
• Flow Cytometry: 5–10 µg/ml

Note: Optimal dilutions should be determined by the end-user based on experimental conditions.