Cost-effective Apoptosis Kit

FITC Annexin V Apoptosis Detection Kit with PI.

FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V FITC serves as a fluorescent probe for apoptotic cells. They will not bind normal and intact cells. However, necrotic cells are leaky enough to pass Annexin V FITC into cytoplasm and provide accessibility for binding to inner membrane phosphatidylserine (false positive apoptosis). Indeed, apoptotic cells have to be differentiated from necrotic cells by PI-stained apoptotic nuclei in comparison to PI-stained necrotic nuclei. But this assay due to its distinctive design is probably able to distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway. The dead cells will probably stain only with both PI. However, when apoptosis is measured over time, cells can be often tracked from FITC Annexin V and PI negative (viable, or no measurable apoptosis), to FITC Annexin V positive and PI negative (early apoptosis, membrane integrity is yet present) and finally to FITC Annexin V and PI positive (end stage apoptosis). In fact, the movement of cells through these three stages suggests apoptosis.