Mouse Anti-Human Beta-Actin

• Beta-actin is a highly conserved cytoskeletal protein expressed ubiquitously in human cells. It plays a crucial role in:

  • Maintaining cell structure and motility.
  • Forming structural filaments (F-actin) through the polymerization of globular actin (G-actin).
  • Regulating cytoskeletal dynamics.

  Beta-actin is commonly used as a loading control in protein detection assays, such as Western blotting, due to its consistent expression levels.

• • Product Number: PDZMM114-F
  • Host Species: Mouse
  • Target Species: Human, Horse, Rat, Dog, Bovine, Monkey
  • Conjugation: FITC (Fluorescein isothiocyanate)
  • Clonality: Monoclonal
  • Isotype: IgG
  • Form: Liquid

  Immunogen

  The immunogen is a KLH-conjugated synthetic peptide derived from the N-terminal region of Beta-actin protein, ensuring specificity and high-quality binding.

  Purification

  The antibody is purified via immunoaffinity chromatography using an immunogen peptide coupled to Sepharose 4B, ensuring high specificity and purity.

• • Storage Instructions:
    • Store at 4°C.
    • Avoid freezing.
    • Protect from light to preserve FITC stability.
  • Storage Buffer:
    • Phosphate-buffered saline (pH 7.4) containing stabilizer and ≤0.09% sodium azide.

  This product is stable as an undiluted liquid at 4°C until the expiry date. For best results, dilute only immediately before use. If the product appears cloudy after standing at room temperature, centrifuge prior to use.

This antibody is suitable for the following research applications:

• Flow Cytometry
• Immunocytochemistry (ICC)
• Immunohistochemistry (IHC)

Recommended starting dilutions:

• IHC (Paraffin): 5–10 µg/ml
• IHC (Frozen): 5–10 µg/ml
• Immunofluorescence: 5–10 µg/ml
• Flow Cytometry: 5–10 µg/ml

Note: Optimal dilutions should be determined by the end user based on experimental conditions.