Mouse Anti-Human TIE1

• TIE1 (Tyrosine kinase with immunoglobulin-like and EGF-like domains 1) is a cell surface protein predominantly expressed in endothelial cells, with additional expression in immature hematopoietic cells and platelets. It plays a key role in angiogenesis and vascular homeostasis. TIE1 regulates cell adhesion molecules (CAMs), such as VCAM-1, E-selectin, and ICAM-1, through a p38-dependent mechanism. TIE1’s expression enhances the attachment of monocyte-derived immune cells to endothelial cells, and it is associated with proinflammatory effects. TIE1 may play a role in endothelial inflammatory diseases like atherosclerosis, making it a target of interest in vascular disease research.

• • Form: Liquid
  • Host Species: Mouse
  • Target Species: Human
  • Conjugation: Unconjugated
  • Clonality: Monoclonal
  • Isotype: IgG

  Purification

  This antibody is purified via immunoaffinity chromatography, using an immunogen peptide derived from the TIE1protein, coupled to Sepharose 4B.

• • • Storage Instructions: Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. The product is stable for several weeks at 4°C as an undiluted liquid.
  • Storage Buffer: Phosphate buffered saline (pH 7.4), contains stabilizer and ≤0.09% sodium azide.

  For extended storage, aliquot and store at -20°C or below. Centrifuge if product is not completely clear after standing at room temperature.

This antibody is suitable for the following research applications:

• Western Blotting (WB)
• Immunohistochemistry (IHC-P, IHC-Frozen)
• Dot Blot
• Immunomicroscopy
• Immunofluorescence (IF)
• Flow Cytometry (FCM)

Recommended starting dilutions:

• Western Blotting: 3-5 µg/ml
• IHC (Paraffin/Frozen): 5-10 µg/ml
• Immunofluorescence: 5-10 µg/ml
• Flow Cytometry: 5-10 µg/ml

Note: Optimal dilutions should be determined by the end-user based on experimental conditions.