Mouse Anti-Human OCT4

• OCT4 is a transcription factor critically involved in maintaining the pluripotency of embryonic stem cells (ESCs). It is often used as a marker to identify undifferentiated stem cells. The expression of OCT4 must be tightly regulated, as excess or insufficient expression of OCT4 leads to differentiation, causing loss of stem cell properties.

• Product Details

  • Host Species: Mouse
  • Target Species: Human
  • Conjugation: Unconjugated
  • Clonality: Monoclonal
  • Isotype: IgG
  • Form: Liquid

  Purification

  This antibody is purified via immunoaffinity chromatography, using an immunogen peptide derived from the OCT4protein, coupled to Sepharose 4B.

• • Storage Instructions: Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. The product is stable for several weeks at 4°C as an undiluted liquid.
  • Storage Buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, and 50% glycerol.

  For extended storage, aliquot and store at -20°C or below. Centrifuge if product is not completely clear after standing at room temperature.

This antibody is suitable for the following research applications:

• Western Blotting (WB)
• Immunohistochemistry (IHC-P, IHC-Frozen)
• Dot Blot
• Immunomicroscopy
• Immunofluorescence (IF)
• Flow Cytometry (FCM)

Recommended starting dilutions:

• Western Blotting: 3-5 µg/ml
• IHC (Paraffin/Frozen): 5-10 µg/ml
• Immunofluorescence: 5-10 µg/ml
• Flow Cytometry: 5-10 µg/ml

Note: Optimal dilutions should be determined by the end-user based on experimental conditions.