Mouse Anti-Human Sortilin

• Sortilin (SORT1) is a member of the Vps10p family of sorting receptors. It mediates protein transport between the Golgi apparatus, endosomes, lysosomes, and plasma membrane. Functionally, Sortilin has been implicated in a wide array of biological processes, such as:

  • Glucose and lipid metabolism
  • Neural development
  • Programmed cell death

  Furthermore, Sortilin plays a role in the progression of several human diseases, including:

  • Atherosclerosis and coronary artery disease: It influences lipid metabolism and cardiovascular health.
  • Alzheimer’s disease: It is linked to amyloid precursor protein transport.
  • Cancer: It regulates tumor progression pathways.

  These multifaceted roles make Sortilin a vital target in research across various fields, including oncology, neuroscience, and cardiovascular studies.

• • Host Species: Mouse
  • Target Species: Human
  • Conjugation: Unconjugated
  • Clonality: Monoclonal
  • Isotype: IgG
  • Form: Liquid

  Purification

  This antibody is purified via immunoaffinity chromatography using  a KLH-conjugated synthetic peptide derived from the human Neurotensin receptor type 3 (Sortilin) protein. The immunogen peptide was coupled to Sepharose 4B for enhanced purification.

• • Storage Instructions:
    • Store at -20°C upon receipt.
    • Avoid repeated freeze-thaw cycles.
    • The product is stable for several weeks at 4°C as an undiluted liquid.
  • Storage Buffer:
    • Phosphate buffered saline (pH 7.4) containing stabilizer and ≤0.09% sodium azide.

  For extended storage, aliquot and freeze at -20°C or below. If the product is not completely clear after standing at room temperature, centrifuge before use.

This antibody is suitable for the following research applications:

• Western Blotting (WB)
• Immunohistochemistry (Paraffin/Frozen) (IHC-P/IHC-F)
• Immunofluorescence (IF)
• Dot Blot
• Flow Cytometry (FCM)
• Immunomicroscopy

Recommended starting dilutions:

• Western Blotting: 3–5 µg/ml
• IHC (Paraffin/Frozen): 5–10 µg/ml
• Immunofluorescence: 5–10 µg/ml
• Flow Cytometry: 5–10 µg/ml

Note: Optimal dilutions should be determined by the end-user based on experimental conditions.