Mouse Anti-Human Beta-Actin

• Protein Background: Provide a concise description of Fibromodulin, e.g., “Fibromodulin, encoded by the FMOD gene, is a 42kDa protein and a member of the leucine-rich repeat proteoglycans (SLRPs). It plays a role in cell growth and differentiation and shares sequence homology with biglycan and decorin.”—Briefly explain if the antibody is specialized or optimized, highlighting any unique manufacturing processes that enhance quality or effectiveness (Production Background).
• Immunogen: Describe immunogen used, e.g., “A KLH-conjugated synthetic peptide from human Fibromodulin.”

• Purification: This product was prepared using immunoaffinity chromatography with an immunogen peptide derived from the N-terminal region of human β-actin, coupled to Sepharose 4B.

• Storage & Handling: Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. The product is stable for several weeks at 4°C as an undiluted liquid.
• Storage Buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, and 50% glycerol. For extended storage, aliquot and store at -20°C or below. Centrifuge if product is not completely clear after standing at room temperature.

Suitability Applications: 

• • Western Blotting (WB)
  • Immunohistochemistry (IHC-P, IHC-Frozen)
  • Dot Blot
  • Immunomicroscopy
  • Immunofluorescence
  • Flow Cytometry
  • ELISA

Application Notes:

• Optimal dilutions should be determined by the end-user based on experimental conditions. Recommend users start with suggested dilutions but adjust as necessary.
  • Western Blotting: 1/2000
  • IHC (Paraffin/Frozen): 1/2000 
• The Mouse Anti-Human Beta-Actin-HRP antibody has received positive feedback from customers. However, if it fails to give results, please contact Padza’s scientific support team for assistance.
• “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.”