FITC Mouse Anti-Human Beta-Actin

Product Datasheet

Mouse Anti-Human Beta-Actin-FITC

Overview

Product number                PDZMM114-F

Host species                     Mouse

Target species                  Human, Horse, Rat, Dog, Bovine, Monkey

Suitable for:                      Flow cytometry, ICC, IHC

Immunogen                       A KLH-conjugated synthetic peptide derived from N-terminal region of Beta- actin protein was used for immunization.

Conjugation                       FITC

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Properties

Form                                  Liquid

Storage instructions        Shipped at 4 °C. Store at 4 °C. Avoid freezing. Store at darkness.

Storage buffer                  Phosphate buffered saline pH 7.4, contains stabilizer and ≤0.09% sodium azide.

Purity                                Immunogen affinity or SpG purified

Purification notes            This product was prepared by immunoaffinity chromatography using immunogen peptide coupled to Sepharose 4B.

Conjugation notes            FITC-conjugated

Clonality                           Monoclonal

Isotype                              IgG

General notes                   Centrifuge product if not completely clear after standing at room temperature. This product is stable before the expiry date at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our scientific support team for assistance.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end-user.

Product Usage Information:

Application Dilutions

Immunohistochemistry (Paraffin)                   5-10 ug/ml

Immunohistochemistry (Frozen)                     5-10 ug/ml

Immunofluorescence                                       5-10 ug/ml

Flow Cytometry                                                5-10 ug/ml

Background:

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ- actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Please note: All products are “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES”

References:

 

  1. Herman, M. (1993) Curr Opin Cell Biol 5, 48-55.
  2. Condeelis, (2001) Trends Cell Biol 11, 288-93.
  3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
  4. Kayalar, et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
  5. Communal, et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
  6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137948/

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