Mouse Anti-E-Cadherin

Product Datasheet

Mouse Anti-E-Cadherin

Overview

Product number                 PDZMM130

Host species                       Mouse

Target species                   Human

Suitable for:                       IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr

 Immunogen                       A KLH-conjugated synthetic peptide derived from human E-Cadherin protein was used for immunization.

Conjugation                        Unconjugated

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Properties

Form                                    Liquid

Storage instructions           Shipped at 4 °C. Store at -20 °C. Avoid freeze/thaw cycle. Please see notes section.

 

Storage buffer                    Phosphate buffered saline pH 7.4, contains stabilizer and ≤0.09% sodium azide.

 

Purity                                 immunogen affinity or SpG purified

Purification notes             This product was prepared by immunoaffinity chromatography using immunogen peptide coupled to Sepharose 4B.

Conjugation notes             –

Clonality                            Monoclonal

Isotype                              IgG

General notes                   For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our scientific support team for assistance.

 

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end-user.

Product Usage Information:

Application Dilutions

Western Blotting                                               3-5 ug/ml

Immunohistochemistry (Paraffin)                    5-10 ug/ml

Immunohistochemistry (Frozen)                      5-10 ug/ml

Immunofluorescence                                        5-10 ug/ml

Flow Cytometry                                                 5-10 ug/ml

 

Please note: All products are “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES”

 

Background:

Cadherin-1 (not to be confused with the APC/C activator protein CDH1) also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene.

Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer. When compared with invasive ductal carcinoma, E-cadherin expression is markedly reduced or absent in the great majority of invasive lobular carcinomas when studied by immunohistochemistry.

Terms and conditions

Guarantee only valid for products bought direct from PADZA or one of our authorized distributors

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 Note: This product has originally been developed at Avicenna Research Institute, Tehran, IRAN and assigned to PADZA Company according to contract 98/15/191dated 98/01/10.