Mouse Anti-Human Ki67

Product Datasheet

Mouse Anti- Human Ki67

Overview

Product number                   PDZMM131

Host species                        Mouse

Target species                     Human

Suitable for:                         IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr

Immunogen                          A KLH-conjugated synthetic peptide derived from human Ki67 protein was used for immunization.

Conjugation                          Unconjugated

 

Properties

Form                                     Liquid

Storage instructions            Shipped at 4 °C. Store at -20 °C. Avoid freeze/thaw cycle. Please see notes section.

 

Storage buffer                     Phosphate buffered saline pH 7.4, contains stabilizer and ≤0.09% sodium azide.

 

Purity                                   immunogen affinity or SpG purified

Purification notes               This product was prepared by immunoaffinity chromatography using immunogen peptide coupled to Sepharose 4B.

Conjugation notes               –

Clonality                              Monoclonal

Isotype                                IgG

General notes                     For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our scientific support team for assistance.

 

 

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end-user.

Product Usage Information:

Application Dilutions

Western Blotting                                              3-5 ug/ml

Immunohistochemistry (Paraffin)                   5-10 ug/ml

Immunohistochemistry (Frozen)                     5-10 ug/ml

Immunofluorescence                                       5-10 ug/ml

Flow Cytometry                                                5-10 ug/ml

 

Please note: All products are “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES”

 

Background:

Antigen KI-67 also known as Ki-67 or MKI67 (Marker Of Proliferation Ki-67) is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67).

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle.[13] In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy. Ki-67 is an excellent marker to determine the growth fraction of a given cell population.

 

Terms and conditions

Guarantee only valid for products bought direct from PADZA or one of our authorized distributors

 

 

 Note: This product has originally been developed at Avicenna Research Institute, Tehran, IRAN and assigned to PADZA Company according to contract 98/15/191dated 98/01/10.