Mouse Anti-Human Sox2

• Sox2 is a critical transcription factor for pluripotency maintenance in embryonic stem cells. It also plays a crucial role in the self-renewal of neural stem cells. Sox2 belongs to the Sox family, which contains highly conserved HMG box domains, and is involved in mammalian development. Given its role in induced pluripotency, Sox2 is essential for regenerative medicine and stem cell research.

• • Form: Liquid
  • Host Species: Mouse
  • Target Species: Human
  • Conjugation: Unconjugated
  • Clonality: Monoclonal
  • Isotype: IgG

  Purification

  This antibody is purified via immunoaffinity chromatography, using an immunogen peptide derived from the Sox2protein, coupled to Sepharose 4B.

• • Storage Instructions: Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. The product is stable for several weeks at 4°C as an undiluted liquid.
  • Storage Buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, and 50% glycerol.

  For extended storage, aliquot and store at -20°C or below. Centrifuge if product is not completely clear after standing at room temperature.

This antibody is suitable for the following research applications:

• Western Blotting (WB)
• Immunohistochemistry (IHC-P, IHC-Frozen)
• Dot Blot
• Immunomicroscopy
• Immunofluorescence (IF)
• Flow Cytometry (FCM)

Recommended starting dilutions:

• Western Blotting: 3-5 µg/ml
• IHC (Paraffin/Frozen): 5-10 µg/ml
• Immunofluorescence: 5-10 µg/ml
• Flow Cytometry: 5-10 µg/ml

Note: Optimal dilutions should be determined by the end-user based on experimental conditions.