Mouse Anti-Human Nanog

• Nanog is a transcription factor that plays a crucial role in maintaining pluripotency in embryonic stem cells (ESCs). It inhibits cell differentiation by suppressing cell determination factors. In addition to its key function in stem cell biology, Nanog is associated with various types of cancer due to its involvement in stem cell self-renewal and tumorigenesis.

• Host Species: Mouse
• Target Species: Human
• Conjugation: Unconjugated
• Clonality: Monoclonal
• Isotype: IgG

• Form: Liquid

Purification

This antibody is purified via immunoaffinity chromatography, using an immunogen peptide derived from the Nanogprotein, coupled to Sepharose 4B.

• • Storage Instructions: Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. The product is stable for several weeks at 4°C as an undiluted liquid.
  • Storage Buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, and 50% glycerol.

  For extended storage, aliquot and store at -20°C or below. Centrifuge if product is not completely clear after standing at room temperature.

This antibody is suitable for the following research applications:

• Western Blotting (WB)
• Immunohistochemistry (IHC-P, IHC-Frozen)
• Dot Blot
• Immunomicroscopy
• Immunofluorescence (IF)
• Flow Cytometry (FCM)

Recommended starting dilutions:

• Western Blotting: 3-5 µg/ml
• IHC (Paraffin/Frozen): 5-10 µg/ml
• Immunofluorescence: 5-10 µg/ml
• Flow Cytometry: 5-10 µg/ml

Note: Optimal dilutions should be determined by the end-user based on experimental conditions.