Product Name: Mouse Anti-Human Beta-Actin |
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Product Number: PDZMM114 |
Clonality: Monoclonal |
Conjugation: Unconjugated |
Host Species: Mouse |
Target Species: Human |
Mouse Anti-Human Beta-Actin
Beta-actin, a non-muscle cytoskeletal protein, is essential for maintaining the structure and motility of eukaryotic cells. It plays a key role in cell movement, division, and integrity. Beta-actin, along with gamma-actin, coexists in most cell types and forms part of the cytoskeleton. Due to its ubiquitous expression in all eukaryotic cells, it is frequently used as a loading control in protein assays, particularly Western blotting. Beta-actin is often used in cancer research and cell biology to assess the intracellular distribution of actin and its role in various cellular processes, including cytokinesis, stress responses, and apoptotic conditions.
Properties
- Form: Liquid
- Storage Instructions:
- Shipped at 4 °C.
- Store at -20 °C. Avoid freeze/thaw cycles.
- For extended storage, aliquot contents and freeze at -20 °C or below.
- Stable for several weeks at 4 °C as an undiluted liquid.
- Storage Buffer:
- Phosphate-buffered saline (pH 7.4), contains stabilizer and ≤0.09% sodium azide
- Purity: Immunogen affinity or SpG purified
- Purification Notes: Prepared by immunoaffinity chromatography using immunogen peptide coupled to Sepharose 4B
- Clonality: Monoclonal
- Isotype: IgG
General Notes
- Centrifuge product if not completely clear after standing at room temperature.
- Dilute only prior to immediate use.
- For research use only. Not for diagnostic procedures.
- Customer feedback indicates excellent performance. If results are unsatisfactory, contact scientific support for assistance.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, and 50% glycerol. Store at –20°C. After receiving, aliquot the antibody and store at –20°C.
Terms and Conditions
Guarantee valid only for products purchased directly from PADZA or from authorized distributors.
Tested Applications:
- Western Blotting (WB): 3-5 µg/mL
- Immunohistochemistry (Paraffin, IHC-P): 5-10 µg/mL
- Immunohistochemistry (Frozen, IHC-Fr): 5-10 µg/mL
- Immunofluorescence: 5-10 µg/mL
- Flow Cytometry: 5-10 µg/mL
Application Notes:
- The optimal dilution/concentration should be determined by the end-user based on experimental conditions.
Overview
Mouse Anti-Human Beta-Actin is a monoclonal antibody targeting the beta-actin protein, which is a key component of the cytoskeleton in eukaryotic cells. Beta-actin is involved in cell motility, structure, and integrity. It is ubiquitously expressed in all human cells and is commonly used as a loading control in protein assays, including Western Blotting, due to its consistent expression. This antibody is ideal for studying the intracellular distribution of beta-actin and its role in cell structure dynamics.
- Published Studies: We will update this section with studies where this antibody was successfully used. (“This antibody was featured in Journal XYZ, 2023, in studies on targeted immunotherapy.”)
References
- Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
- Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
- Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
- Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
- Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
- Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.
PMC Article
(You can view the article referenced in the NCBI: PMC4137948)
- Case Studies: Brief summaries of key research projects or notable outcomes demonstrating the product’s effectiveness.
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Publications & Research
Ordering Information
Sizes and Packaging Options: Various sizes (50 µL, 100 µL) and larger quantities are available.
Sizes | Ordering Code | Packaging | Availability |
50 µL | PDZMM101-50 | Standard vial | Order Now |
100 µL | PDZMM101-100 | Standard vial | Order Now |
💬 Request a Quote for Bulk Purchases
PADZA ensures product performance when purchased directly from PADZA or authorized distributors. Contact our scientific support team for any technical issues.
📩 Contact our Scientific Support Team for application-specific queries or troubleshooting.
Terms & Conditions: This product is guaranteed only when purchased directly from PADZA or its authorized distributors.
Contact Padza for Product Support and Inquiries
At Padza, we are committed to providing exceptional support for our products and services. Whether you have feedback, need assistance with an application, or require technical troubleshooting, our team is here to help. Please fill out the form below to get in touch with us, and our technical experts will respond promptly to assist with your specific needs. We look forward to supporting your research and ensuring the success of your projects.
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