FITC Mouse Anti-Human CD14

Product Datasheet

Mouse Anti-Human CD14-FITC

Overview

Product number                  PDZMM101-F

Host species                       Mouse

Target species                    Human

Suitable for:                        Flow cytometry, ICC, IHC-Fr, IHC-P

Immunogen                        A KLH-conjugated synthetic peptide derived from human CD14 protein was used for immunization.

Conjugation                       FITC

 

Properties

Form                                  Liquid

Storage instructions         Shipped at 4 °C. Store at 4 °C. Avoid freezing. Store at darkness.

 

Storage buffer                   Phosphate buffered saline pH 7.4, contains stabilizer and ≤0.09% sodium azide.

Purity                              SpG purified

Purification notes         This product was prepared by immunoaffinity chromatography using protein G coupled to Sepharose 4B.

Conjugation notes        FITC-conjugated

Clonality                      Monoclonal

Isotype                         IgG

General notes              Centrifuge product if not completely clear after standing at room temperature. This product is stable before the expiry date at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our scientific support team for assistance.

 

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end-user.

Product Usage Information:

Application Dilutions

Immunohistochemistry (Paraffin)             5-10 ug/ml

Immunohistochemistry (Frozen)               5-10 ug/ml

Immunofluorescence                                     5-10 ug/ml

Flow Cytometry                                                5-10 ug/ml

 

 

Background:

CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP).

CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol (GPI) tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).

 

Please note: All products are “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES”

Note: This product has originally been developed at Avicenna Research Institute, Tehran, IRAN and assigned to PADZA Company according to contract 98/15/191, dated 98/01/10.