Mouse Anti-Human Fibromodulin

Product Datasheet

Mouse Anti-Human Fibromodulin

Overview

Product number                 PDZMM121

Host species                      Mouse

Target species                   Human

Suitable for:                       IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr

Immunogen                        A KLH-conjugated synthetic peptide derived from human Fibromodulin protein was used for immunization.

Conjugation                        Unconjugated

 

Properties

Form                                     Liquid

Storage instructions            Shipped at 4 °C. Store at -20 °C. Avoid freeze/thaw cycle. Please see notes section.

 

Storage buffer                   Phosphate buffered saline pH 7.4, contains stabilizer and ≤0.09% sodium azide.

Purity                                 Immunogen affinity purified

Purification notes             This product was prepared by immunoaffinity chromatography using immunogen peptide coupled to Sepharose 4B.

Conjugation notes             –

Clonality                            Monoclonal

Isotype                              IgG

General notes                   For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our scientific support team for assistance.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end-user.

Product Usage Information:

Application Dilutions

Western Blotting                                               3-5 ug/ml

Immunohistochemistry (Paraffin)                    5-10 ug/ml

Immunohistochemistry (Frozen)                      5-10 ug/ml

Immunofluorescence                                        5-10 ug/ml

Flow Cytometry                                                 5-10 ug/ml

 

Please note: All products are “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES”

 

Background:

Fibromodulin is a protein that in humans is encoded by the FMOD gene.

Fibromodulin is a 42kDa protein of a family of small interstitial leucine-rich repeat proteoglycans (SLRPs). It can have up to four N-linked keratan sulfate chains attached to the core protein within the leucine-rich region. It shares significant sequence homology with biglycan and decorin.

References:

The product has been utilized in:

Farahi L, Ghaemimanesh F, Milani S, Razavi SM, Hadavi R, Bayat AA, Salimi A, Akhondi MM, Rabbani H. GPI-anchored fibromodulin as a novel target in chronic lymphocytic leukemia: diagnostic and therapeutic implications. Iranian Journal of Immunology. 2019 Jun 1;16(2):127-41.

Note: This product has originally been developed at Avicenna Research Institute, Tehran, IRAN and assigned to PADZA Company according to contract 98/15/191dated 98/01/10.